Article thumbnail
Location of Repository

Mapping and cloning of a fla-che region of the Rhizobium meliloti chromosome.

By R J Ziegler, C Peirce and K Bergman

Abstract

We constructed a genetic map of the fla-che region of the Rhizobium meliloti chromosome using cotransduction with bacteriophage phi M12. Several other chromosomal markers located in the general area are included in the map. We isolated plasmids carrying wild-type DNA inserts that complement the mapped mutations from a genomic library carried in the broad-host-range vector pLAFR1. The complementation data obtained from the clones confirmed the contransduction map and clarified the exact order of several of the behavioral genes. A restriction map of this area was developed by using the cloned DNA. One of the five individual EcoRI fragments subcloned from the original clones complemented two of the behavioral mutations

Topics: Research Article
Year: 1986
DOI identifier: 10.1128/jb.168.2.785-790.1986
OAI identifier: oai:pubmedcentral.nih.gov:213552
Provided by: PubMed Central
Sorry, our data provider has not provided any external links therefore we are unable to provide a link to the full text.

Suggested articles


To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.