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Parallel gene analysis with allele-specific padlock probes and tag microarrays

By Johan Banér, Anders Isaksson, Erik Waldenström, Jonas Jarvius, Ulf Landegren and Mats Nilsson

Abstract

Parallel, highly specific analysis methods are required to take advantage of the extensive information about DNA sequence variation and of expressed sequences. We present a scalable laboratory technique suitable to analyze numerous target sequences in multiplexed assays. Sets of padlock probes were applied to analyze single nucleotide variation directly in total genomic DNA or cDNA for parallel genotyping or gene expression analysis. All reacted probes were then co-amplified and identified by hybridization to a standard tag oligonucleotide array. The technique was illustrated by analyzing normal and pathogenic variation within the Wilson disease-related ATP7B gene, both at the level of DNA and RNA, using allele-specific padlock probes

Topics: NAR Methods Online
Publisher: Oxford University Press
Year: 2003
DOI identifier: 10.1093/nar/gng104
OAI identifier: oai:pubmedcentral.nih.gov:212823
Provided by: PubMed Central
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