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Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2)

By Hatice Akarsu, Wilhelm P. Burmeister, Carlo Petosa, Isabelle Petit, Christoph W. Müller, Rob W.H. Ruigrok and Florence Baudin

Abstract

During influenza virus infection, viral ribonucleoproteins (vRNPs) are replicated in the nucleus and must be exported to the cytoplasm before assembling into mature viral particles. Nuclear export is mediated by the cellular protein Crm1 and putatively by the viral protein NEP/NS2. Proteolytic cleavage of NEP defines an N-terminal domain which mediates RanGTP-dependent binding to Crm1 and a C- terminal domain which binds to the viral matrix protein M1. The 2.6 Å crystal structure of the C-terminal domain reveals an amphipathic helical hairpin which dimerizes as a four-helix bundle. The NEP–M1 interaction involves two critical epitopes: an exposed tryptophan (Trp78) surrounded by a cluster of glutamate residues on NEP, and the basic nuclear localization signal (NLS) of M1. Implications for vRNP export are discussed

Topics: Articles
Publisher: Oxford University Press
Year: 2003
DOI identifier: 10.1093/emboj/cdg449
OAI identifier: oai:pubmedcentral.nih.gov:212717
Provided by: PubMed Central
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