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Coordinate regulation of phospholipid biosynthesis by serine in Saccharomyces cerevisiae.

By M J Homann, A M Bailis, S A Henry and G M Carman


The addition of L-serine to inositol-containing growth medium repressed membrane-associated CDPdiacylglycerol synthase (CTP:phosphatidate cytidylyltransferase, EC and phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase, EC activities and subunit levels in wild-type Saccharomyces cerevisiae. Enzyme activities and subunit levels were not repressed when inositol was absent from the growth medium. The addition of L-serine to the growth medium did not affect the phospholipid composition of wild-type cells. CDPdiacylglycerol synthase and phosphatidylserine synthase were not regulated in the S. cerevisiae inositol biosynthesis ino2, ino4, and opi1 regulatory mutants, suggesting that regulation by inositol plus L-serine is coupled to inositol synthesis. Inositol and L-serine did not affect the activities of purified CDPdiacylglycerol synthase and phosphatidylserine synthase. The addition of compounds structurally related to L-serine to the growth medium of wild-type cells also resulted in a repression of CDPdiacylglycerol synthase and phosphatidylserine synthase but only in the presence of inositol. Phosphatidylinositol synthase (CDPdiacylglycerol:myo-inositol 3-phosphatidyltransferase, EC was not regulated by inositol plus L-serine

Topics: Research Article
Year: 1987
DOI identifier: 10.1128/jb.169.7.3276-3280.1987
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Provided by: PubMed Central
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