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Cloning of the Escherichia coli K-12 hemB gene.

By J M Li, H Umanoff, R Proenca, C S Russell and S D Cosloy

Abstract

An Escherichia coli heme-requiring, heme-permeable mutant had no detectable 5-aminolevulinate dehydratase or porphobilinogen deaminase activities. The gene which complemented this mutation was cloned to a high-copy-number plasmid, and porphobilinogen deaminase activity was restored to normal levels, but the synthesis of 5-aminolevulinate dehydratase increased 20- to 30-fold. A maxicell procedure confirmed that the gene cloned was hemB

Topics: Research Article
Year: 1988
DOI identifier: 10.1128/jb.170.2.1021-1025.1988
OAI identifier: oai:pubmedcentral.nih.gov:210762
Provided by: PubMed Central
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