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Genetic mapping of the structural gene for phospholipase C of Pseudomonas aeruginosa PAO.

By V Lindgren, R M Ostroff, M L Vasil and B Wretlind


An insertion mutation constructed by gene replacement methods was used to map the gene corresponding to the hemolytic phospholipase C (plcS gene) in Pseudomonas aeruginosa PAO1 by R68.45-mediated conjugation. plcS mapped approximately at 67 min on the 75-min chromosomal map (B. W. Holloway, K. O'Hoy, and H. Matsumoto, p. 213-221, in S. J. O'Brien, ed., Genetic Maps 1987, vol. 4, 1987), between the markers pur-67 and pru-375 and considerably distal to the regulatory genes plcA and plcB, which are located at approximately 12 min

Topics: Research Article
Year: 1990
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Provided by: PubMed Central
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