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TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene.

By B Sharma and T M Hill

Abstract

We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented to allow transcription of the rcsC gene but prevent DNA replication in the terminus-to-origin direction. Our results demonstrate that the TerF site is functional in both chromosomal and plasmid environments and that the stability of the Tus-TerF protein-DNA complex more closely resembles the plasmid R6K Ter sites than the chromosomal TerB site

Topics: Research Article
Year: 1992
OAI identifier: oai:pubmedcentral.nih.gov:207506
Provided by: PubMed Central
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