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The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons.

By X Liu and P Matsumura

Abstract

The Escherichia coli flhD operon encodes two genes, flhD and flhC. Both gene products were overproduced and purified. The purified proteins formed a complex consisting of two FlhD and two FlhC molecules. Mobility shift assays showed that the FlhD/FlhC complex had a DNA-binding activity and bound to the upstream regions of fliA, flhB, and fliL operons (class II), which are under direct control of the flhD operon. DNase I footprinting analyses of FlhD/FlhC binding to the three class II promoter regions revealed protection of a 48-bp region of the fliA operon between positions -41 to -88, a 50-bp region of the flhB operon between positions -28 to -77, and a 48-bp region of the fliL operon between positions -29 to -76. In vitro transcription experiments demonstrated that the FlhD/FlhC complex is a transcriptional activator required for the transcription of the three class II operons examined in vitro

Topics: Research Article
Year: 1994
DOI identifier: 10.1128/jb.176.23.7345-7351.1994
OAI identifier: oai:pubmedcentral.nih.gov:197124
Provided by: PubMed Central
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