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Gene Expression Analysis Using Oligonucleotide Arrays Produced by Maskless Photolithography

By Emile F. Nuwaysir, Wei Huang, Thomas J. Albert, Jaz Singh, Kate Nuwaysir, Alan Pitas, Todd Richmond, Tom Gorski, James P. Berg, Jeff Ballin, Mark McCormick, Jason Norton, Tim Pollock, Terry Sumwalt, Lawrence Butcher, DeAnn Porter, Michael Molla, Christine Hall, Fred Blattner, Michael R. Sussman, Rodney L. Wallace, Franco Cerrina and Roland D. Green


Microarrays containing 195,000 in situ synthesized oligonucleotide features have been created using a benchtop, maskless photolithographic instrument. This instrument, the Maskless Array Synthesizer (MAS), uses a digital light processor (DLP) developed by Texas Instruments. The DLP creates the patterns of UV light used in the light-directed synthesis of oligonucleotides. This digital mask eliminates the need for expensive and time-consuming chromium masks. In this report, we describe experiments in which we tested this maskless technology for DNA synthesis on glass surfaces. Parameters examined included deprotection rates, repetitive yields, and oligonucleotide length. Custom gene expression arrays were manufactured and hybridized to Drosophila melanogaster and mouse samples. Quantitative PCR was used to validate the gene expression data from the mouse arrays. [The sequence data from this study have been submitted to GEO under accession nos. GPL208, GSM2409, GSM2410, GSM2411, GSM2412, GSM2413, GSM2414, GSE81, GSE82.

Topics: Methods
Publisher: Cold Spring Harbor Laboratory Press
Year: 2002
DOI identifier: 10.1101/gr.362402
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Provided by: PubMed Central
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