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Liquid Nitrogen Cryo-Impacting: a New Concept for Cell Disruption

By Richard A. Smucker and Robert M. Pfister


High-efficiency disruption of bacteria can be accomplished in 2 or more min by the new procedure of liquid nitrogen cryo-impacting. Release of the dipicolinic acid-Ca(2+) chelate paralleled the breakage of Bacillus megaterium endospores. Lactate dehydrogenase activity was much better in supernates from liquid nitrogen cryo-impacting-broken Escherichia coli cells than in those from sonically treated and broken E. coli cells

Topics: Metabolism and Products
Year: 1975
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Provided by: PubMed Central
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