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Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter.

By M Tan and J N Engel

Abstract

Chlamydia trachomatis RNA polymerase was partially purified by heparin-agarose chromatography and used in conjunction with a plasmid-borne G-less cassette template to characterize the C. trachomatis rRNA P1 promoter in vitro. Stepwise mutational analysis revealed that sequences in the -10, -25, and -35 regions are necessary for promoter activity, but no sequence upstream of position -40 is required. Partially purified C. trachomatis RNA polymerase and purified Escherichia coli holoenzyme exhibited some differences in promoter specificity

Topics: Research Article
Year: 1996
DOI identifier: 10.1128/jb.178.23.6975-6982.1996
OAI identifier: oai:pubmedcentral.nih.gov:178601
Provided by: PubMed Central
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