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Promoter analysis of the Xanthomonas campestris pv. campestris gum operon directing biosynthesis of the xanthan polysaccharide.

By F Katzen, A Becker, A Zorreguieta, A Pühler and L Ielpi

Abstract

The Xanthomonas campestris gum gene cluster is composed of 12 genes designated gumB, -C, -D, -E, -F, -G, -H, -I, -J, -K, -L, and -M. The transcriptional organization of this gene cluster was analyzed by the construction of gum-lacZ transcriptional fusions in association with plasmid integration mutagenesis. This analysis, coupled with primer extension assays, indicated that the gum region was mainly expressed as an operon from a promoter located upstream of the first gene, gumB

Topics: Research Article
Year: 1996
DOI identifier: 10.1128/jb.178.14.4313-4318.1996
OAI identifier: oai:pubmedcentral.nih.gov:178194
Provided by: PubMed Central
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