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Bacillus subtilis gnt repressor mutants that diminish gluconate-binding ability.

By K Yoshida, H Ohmori, Y Miwa and Y Fujita


The Bacillus subtilis gnt operon is negatively regulated by GntR, which is antagonized by gluconate. Three GntR mutants with diminished gluconate-binding ability were obtained. Two were missense mutants (Met-209 to Ile and Ser-230 to Leu), whereas the third had a deletion of the C-terminal 23 amino acids. The mutant GntR proteins were unable to become properly detached from the gnt operator even in the presence of gluconate

Topics: Research Article
Year: 1995
DOI identifier: 10.1128/jb.177.16.4813-4816.1995
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Provided by: PubMed Central
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