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Possible role for the essential GTP-binding protein Obg in regulating the initiation of sporulation in Bacillus subtilis.

By S J Vidwans, K Ireton and A D Grossman


We fused obg, encoding an essential GTP-binding protein in Bacillus subtilis, to the LacI-repressible, IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter Pspac. Depletion of Obg, following removal of IPTG, caused a defect in sporulation and in expression of sporulation genes that are activated by Spo0A approximately P. These defects were significantly relieved by a mutation in spo0A (rvtA11) that bypasses the normal phosphorylation pathway, indicating that Obg might normally be required, either directly or indirectly, to stimulate activity of the phosphorelay that activates Spo0A

Topics: Research Article
Year: 1995
DOI identifier: 10.1128/jb.177.11.3308-3311.1995
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Provided by: PubMed Central
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