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Rapid nucleic acid assay for detection of bacteria with tetM-mediated tetracycline resistance.

By R Sanchez-Pescador, J A Running, M M Stempien and M S Urdea

Abstract

A novel nucleic acid assay has been developed to screen bacterial populations for the presence of the tetM structural gene. The method involves the specific hybridization of several synthetic oligonucleotides to the gene in a crude bacterial lysate solution. As few as 1.5 x 10(4) CFU can be detected with the assay

Topics: Research Article
Year: 1989
OAI identifier: oai:pubmedcentral.nih.gov:172761
Provided by: PubMed Central
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