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Multiplex PCR Assay for Identification of Human Diarrheagenic Escherichia coli

By Claudia Toma, Yan Lu, Naomi Higa, Noboru Nakasone, Isabel Chinen, Ariela Baschkier, Marta Rivas and Masaaki Iwanaga

Abstract

A multiplex PCR assay for the identification of human diarrheagenic Escherichia coli was developed. The targets selected for each category were eae for enteropathogenic E. coli, stx for Shiga toxin-producing E. coli, elt and est for enterotoxigenic E. coli, ipaH for enteroinvasive E. coli, and aggR for enteroaggregative E. coli. This assay allowed the categorization of a diarrheagenic E. coli strain in a single reaction tube

Topics: Bacteriology
Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/JCM.41.6.2669-2671.2003
OAI identifier: oai:pubmedcentral.nih.gov:156568
Provided by: PubMed Central
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