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Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification

By Marie L. Landry, Robin Garner and David Ferguson

Abstract

Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells

Topics: Virology
Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/JCM.41.1.346-350.2003
OAI identifier: oai:pubmedcentral.nih.gov:149595
Provided by: PubMed Central
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