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Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Open Reading Frame 4 Protein (Kaposica) Is a Functional Homolog of Complement Control Proteins

By Jayati Mullick, John Bernet, Akhilesh K. Singh, John D. Lambris and Arvind Sahu

Abstract

The genome analysis of Kaposi's sarcoma-associated herpesvirus (KSHV) has revealed the presence of an open reading frame (ORF 4) with sequence homology to complement control proteins. To assign a function to this protein, we have now expressed this ORF using the Pichia expression system and shown that the purified protein inhibited human complement-mediated lysis of erythrocytes, blocked cell surface deposition of C3b (the proteolytically activated form of C3), and served as a cofactor for factor I-mediated inactivation of complement proteins C3b and C4b (the subunits of C3 convertases). Thus, our data indicate that this KSHV inhibitor of complement activation (kaposica) provides a mechanism by which KSHV can subvert complement attack by the host

Topics: Pathogenesis and Immunity
Publisher: American Society for Microbiology
Year: 2003
DOI identifier: 10.1128/JVI.77.6.3878-3881.2003
OAI identifier: oai:pubmedcentral.nih.gov:149522
Provided by: PubMed Central
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