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Local action of long-range repressors in the Drosophila embryo

By Yutaka Nibu, Hailan Zhang and Michael Levine

Abstract

Previous studies have identified two corepressors in the early Drosophila embryo: Groucho and dCtBP. Both proteins are recruited to the DNA template by interacting with short peptide motifs conserved in a variety of sequence-specific transcriptional repressors. Once bound to DNA, Groucho appears to mediate long-range repression, while dCtBP directs short-range repression. The short-range Krüppel repressor was converted into a long-range repressor by replacing the dCtBP interaction motif (PxDLSxH) with a Groucho motif (WRPW). The resulting chimeric repressor causes a different mutant phenotype from that of the native Krüppel protein when misexpressed in transgenic embryos. The different patterning activities can be explained on the basis of long-range silencing within the hairy 5′ regulatory region. The analysis of a variety of synthetic transgenes provides evidence that Groucho-dependent long-range repressors do not always cause the dominant silencing of linked enhancers within a complex cis-regulatory region. We suggest a ‘hot chromatin’ model, whereby repressors require activators to bind DNA

Topics: Article
Publisher: Oxford University Press
Year: 2001
DOI identifier: 10.1093/emboj
OAI identifier: oai:pubmedcentral.nih.gov:125437
Provided by: PubMed Central
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