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Isolation and Genotyping of Helicobacter pylori from Untreated Municipal Wastewater

By Yingzhi Lu, Thomas E. Redlinger, Raquel Avitia, Adriana Galindo and Karen Goodman


For this study, we isolated Helicobacter pylori from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative H. pylori isolates were verified by PCR. Eleven H. pylori isolates were genotyped and fell into four vacA classes: those with the vacA allelic variants s1a and m1, s1b and m1, s2 and m2, or s2 and m1. Most H. pylori isolates were of the vacA s1a/m1 type, which has been shown to be associated with advanced diseases based on genotyping of H. pylori from gastric cancer patients. These results demonstrated that H. pylori survives in water and may be a potential source of H. pylori transmission, especially where water is not adequately treated

Topics: Public Health Microbiology
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/AEM.68.3.1436-1439.2002
OAI identifier: oai:pubmedcentral.nih.gov:123781
Provided by: PubMed Central
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