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Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by XbaI Restriction and Resolved by Pulsed-Field Gel Electrophoresis

By Terri H. Hardwick, Brian Plikaytis, Pamela K. Cassiday, Gary Cage, Mark S. Peppler, Deborah Shea, David Boxrud and Gary N. Sanden

Abstract

The intra- and interlaboratory variabilities of the molecular size measurements of each DNA fragment contributing to three pulsed-field gel electrophoresis (PFGE) profiles were assessed, as were the reproducibilities of the entire PFGE profiles for three Bordetella pertussis strains. The major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. Each PFGE profile was generated reproducibly and was objectively defined by the molecular sizes of its composite fragments. A strain or profile most suitable for use as an internal reference standard was identified

Topics: Epidemiology
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/JCM.40.3.811-816.2002
OAI identifier: oai:pubmedcentral.nih.gov:120225
Provided by: PubMed Central
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