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Microimmunoassay Using a Protein Chip: Optimizing Conditions for Protein Immobilization

By Seung-Yong Seong

Abstract

Optimizing conditions for the microarraying of protein antigens onto glass slides were studied. Various vendors, surface functional groups, buffers, and fixatives were evaluated to enhance protein binding. A total of 125 pg of human immunoglobulin was detectable with this assay system, suggesting that protein microarray can be applied for routine immunodiagnosis

Topics: Experimental Clinical Investigation
Publisher: American Society for Microbiology
Year: 2002
DOI identifier: 10.1128/CDLI.9.4.927-930.2002
OAI identifier: oai:pubmedcentral.nih.gov:120026
Provided by: PubMed Central
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