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Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus

By Rajat Sapra, Marc F. J. M. Verhagen and Michael W. W. Adams

Abstract

Highly washed membrane preparations from cells of the hyperthermophilic archaeon Pyrococcus furiosus contain high hydrogenase activity (9.4 μmol of H2 evolved/mg at 80°C) using reduced methyl viologen as the electron donor. The enzyme was solubilized with n-dodecyl-β-d-maltoside and purified by multistep chromatography in the presence of Triton X-100. The purified preparation contained two major proteins (α and β) in an approximate 1:1 ratio with a minimum molecular mass near 65 kDa and contained ∼1 Ni and 4 Fe atoms/mol. The reduced enzyme gave rise to an electron paramagnetic resonance signal typical of the so-called Ni-C center of mesophilic NiFe-hydrogenases. Neither highly washed membranes nor the purified enzyme used NAD(P)(H) or P. furiosus ferredoxin as an electron carrier, nor did either catalyze the reduction of elemental sulfur with H2 as the electron donor. Using N-terminal amino acid sequence information, the genes proposed to encode the α and β subunits were located in the genome database within a putative 14-gene operon (termed mbh). The deduced sequences of the two subunits (Mbh 11 and 12) were distinctly different from those of the four subunits that comprise each of the two cytoplasmic NiFe-hydrogenases of P. furiosus and show that the α subunit contains the NiFe-catalytic site. Six of the open reading frames (ORFs) in the operon, including those encoding the α and β subunits, show high sequence similarity (>30% identity) with proteins associated with the membrane-bound NiFe-hydrogenase complexes from Methanosarcina barkeri, Escherichia coli, and Rhodospirillum rubrum. The remaining eight ORFs encode small (<19-kDa) hypothetical proteins. These data suggest that P. furiosus, which was thought to be solely a fermentative organism, may contain a previously unrecognized respiratory system in which H2 metabolism is coupled to energy conservation

Topics: Enzymes and Proteins
Publisher: American Society for Microbiology
Year: 2000
OAI identifier: oai:pubmedcentral.nih.gov:101913
Provided by: PubMed Central
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