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Initial Characterization of the Hemolysin Stachylysin from Stachybotrys chartarum

By Stephen J. Vesper, Matthew L. Magnuson, Dorr G. Dearborn, Iwona Yike and Richard A. Haugland

Abstract

Stachybotrys chartarum is a toxigenic fungus that has been associated with human health concerns, including pulmonary hemorrhage and hemosiderosis. This fungus produces a hemolysin, stachylysin, which in its apparent monomeric form has a molecular mass of 11,920 Da as determined by matrix-assisted laser desorption ionization–time of flight mass spectrometry. However, it appears to form polydispersed aggregates, which confounds understanding of the actual hemolytically active form. Exhaustive dialysis or heat treatment at 60°C for 30 min inactivated stachylysin. Stachylysin is composed of about 40% nonpolar amino acids and contains two cysteine residues. Purified stachylysin required more than 6 h to begin lysing sheep erythrocytes, but by 48 h, lysis was complete. Stachylysin also formed pores in sheep erythrocyte membranes

Topics: Molecular Pathogenesis
Publisher: American Society for Microbiology
Year: 2001
DOI identifier: 10.1128/IAI.69.2.912-916.2001
OAI identifier: oai:pubmedcentral.nih.gov:97969
Provided by: PubMed Central
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