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The djlA Gene Acts Synergistically with dnaJ in Promoting Escherichia coli Growth

By Pierre Genevaux, Françoise Schwager, Costa Georgopoulos and William L. Kelley

Abstract

The DnaK chaperone of Escherichia coli is known to interact with the J domains of DnaJ, CbpA, and DjlA. By constructing multiple mutants, we found that the djlA gene was essential for bacterial growth above 37°C in the absence of dnaJ. This essentiality depended upon the J domain of DjlA but not upon the normal membrane location of DjlA

Topics: Genetics and Molecular Biology
Publisher: American Society for Microbiology
Year: 2001
DOI identifier: 10.1128/JB.183.19.5747-5750.2001
OAI identifier: oai:pubmedcentral.nih.gov:95468
Provided by: PubMed Central
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