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16S Ribosomal DNA Terminal Restriction Fragment Pattern Analysis of Bacterial Communities in Feces of Rats Fed Lactobacillus acidophilus NCFM

By Christopher W. Kaplan, Johanna C. Astaire, Mary Ellen Sanders, Bandaru S. Reddy and Christopher L. Kitts

Abstract

16S ribosomal DNA terminal restriction fragment patterns from rat fecal samples were analyzed to track the dynamics of Lactobacillus acidophilus NCFM and discern bacterial populations that changed during feeding with NCFM. Lactobacillus johnsonii and Ruminococcus flavefaciens were tentatively identified as such bacterial populations. The presence of L. johnsonii was confirmed by isolation from feces

Topics: Microbial Ecology
Publisher: American Society for Microbiology
Year: 2001
DOI identifier: 10.1128/AEM.67.4.1935-1939.2001
OAI identifier: oai:pubmedcentral.nih.gov:92815
Provided by: PubMed Central
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