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Haemophilus influenzae pili are composite structures assembled via the HifB chaperone.

By J W St Geme, J S Pinkner, G P Krasan, J Heuser, E Bullitt, A L Smith and S J Hultgren

Abstract

Haemophilus influenzae is a Gram-negative bacterium that represents a common cause of human disease. Disease due to this organism begins with colonization of the upper respiratory mucosa, a process facilitated by adhesive fibers called pili. In the present study, we investigated the structure and assembly of H. influenzae pili. Examination of pili by electron microscopy using quick-freeze, deep-etch and immunogold techniques revealed the presence of two distinct subassemblies, including a flexible two-stranded helical rod comprised of HifA and a short, thin, distal tip structure containing HifD. Genetic and biochemical studies demonstrated that the biogenesis of H. influenzae pili is dependent on a periplasmic chaperone called HifB, which belongs to the PapD family of immunoglobulin-like chaperones. HifB bound directly to HifA and HifD, forming HifB-HifA and HifB-HifD complexes, which were purified from periplasmic extracts by ion-exchange chromatography. Continued investigation of the biogenesis of H. influenzae pili should provide general insights into organelle development and may suggest novel strategies for disease prevention

Topics: Research Article
Year: 1996
DOI identifier: 10.1073/pnas.93.21.11913
OAI identifier: oai:pubmedcentral.nih.gov:38158
Provided by: PubMed Central
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