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Protein phosphatase 2A is required for the initiation of chromosomal DNA replication

By Xiao-Hong Lin, Johannes Walter, Karl Scheidtmann, Kim Ohst, John Newport and Gernot Walter


Protein phosphatase 2A (PP2A) is an abundant, multifunctional serine/threonine-specific phosphatase that stimulates simian virus 40 DNA replication. The question as to whether chromosomal DNA replication also depends on PP2A was addressed by using a cell-free replication system derived from Xenopus laevis eggs. Immunodepletion of PP2A from Xenopus egg extract resulted in strong inhibition of DNA replication. PP2A was required for the initiation of replication but not for the elongation of previously engaged replication forks. Therefore, the initiation of chromosomal DNA replication depends not only on phosphorylation by protein kinases but also on dephosphorylation by PP2A

Topics: Biological Sciences
Publisher: The National Academy of Sciences
Year: 1998
OAI identifier:
Provided by: PubMed Central
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