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smg mutants affect the expression of alternatively spliced SR protein mRNAs in Caenorhabditis elegans

By Mike Morrison, Kevin S. Harris and Mark B. Roth

Abstract

The expression of alternatively spliced mRNAs from genes is an ubiquitous phenomenon in metazoa. A screen for trans-acting factors that alter the expression of alternatively spliced mRNAs reveals that the smg genes of Caenorhabditis elegans participate in this process. smg genes have been proposed to function in degradation of nonsense mutant mRNAs. Here we show that smg genes affect normal gene expression by modulating the levels of alternatively spliced SRp20 and SRp30b mRNAs. These SR genes contain alternatively spliced exons that introduce upstream stop codons. The effect of smg genes on SR transcripts is specific, because the gene encoding the catalytic subunit of the cAMP-dependent protein kinase, which also contains an alternatively spliced exon that introduces upstream stop codon, is not effected in a smg background. These results suggest that the levels of alternatively spliced mRNAs may, in part, be regulated by alternative mRNA stability

Topics: Biological Sciences
Publisher: The National Academy of Sciences of the USA
Year: 1997
DOI identifier: 10.1073/pnas.94.18.9782
OAI identifier: oai:pubmedcentral.nih.gov:23268
Provided by: PubMed Central
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