A simple and fast protocol for the protein complex immunoprecipitation (Co-IP) of effector : host protein complexes

Abstract

Plant pathogens are responsible for enormous damage in natural and cultured ecosystems. One strategy most pathogenic organisms follow is the secretion of effector proteins that manipulate the host immune system to suppress defense responses. There is considerable interest in finding host targets of pathogen effectors as this helps to shape our understanding of how those proteins work in planta. The presented protocol describes a protein complex immunoprecipitation method aimed at verifying protein–protein interactions derived from protein complementation assays like Yeast-two-Hybrid