Skip to main content
Article thumbnail
Location of Repository

Efeitos de meio de cultura, fontes de carbono e nitrogênio, pH e regime luminoso no crescimento de Mycosphaerella musicola Effects of medium, carbon and nitrogen source, pH and light on the growth of Mycosphaerella musicola

By Angélica Virgínia Valois Montarroyos, Rildo Sartori Barbosa Coelho, Gabriela de Morais Guerra Ferraz, Rômulo dos Santos, Venézio Felipe dos Santos and Paulo Paes de Andrade

Abstract

Este trabalho objetivou o estabelecimento de condições favoráveis ao crescimento micelial de M. musicola in vitro, pela avaliação em quatro experimentos, da influência de diferentes meios de cultura (BDA, BDA/IFB, V8, V8/IFB, V8/CaCO3 e V8/CaCO3/IFB); combinações de fontes de carbono (dextrose, maltose, sacarose e xilose) e nitrogênio (peptona, glicina, nitrato de potássio e de sódio); valores de pH (6,8; 6,4; 5,7 e 4,9) e regimes luminosos (escuro contínuo, alternância luminosa e claro contínuo). Observou-se um maior crescimento de M. musicola quando cultivado nos meios de cultura BDA/IFB, V8/IFB e BDA. As fontes de carbono sacarose, maltose e dextrose quando combinadas com a peptona como fonte de nitrogênio, promoveram um maior crescimento micelial de M. musicola. O meio de cultura BDA/IFB, com o valor final de pH ajustado para 5,7, em regime de escuro contínuo, apresentou-se como o melhor para o crescimento de M. musicola.<br>This work aimed the establishment of the best growth conditions of M. musicola mycelia in vitro through the analysis of the influence of different culture media (BDA, BDA/IFB, V8, V8/IFB, V8/CaCO3 and V8/CaCO3/IFB), combinations of carbon (dextrose, maltose, sucrose and xylose) and nitrogen (peptone, glycine, potassium nitrate and sodium nitrate) sources, medium pH values (6.8, 6.4, 5.7 and 4.9) and photoperiods (continuous darkness, alternating darkness/lightness and continuous lightness). At the end of the evaluation period, cultural parameters and the dry weights of colonies were annotated. BDA/IFB, V8/IFB and BDA culture media promoted the best mycelial growth. The experiments also demonstrated that dextrose and sucrose when combined with peptose as a nitrogen source, are the best carbon sources as they promoted the most vigorous mycelial growth. The use of BDA/IFB culture medium, with its final pH adjusted to 5.7, and a photoperiod of continuous darkness was the best condition for the growth of M. musicola

Topics: Pseudocercospora musae, mal-de-sigatoka, sigatoka amarela, banana, Pseudocercospora musae, sigatoka disease, yellow sigatoka, banana, LCC:Botany, LCC:QK1-989, LCC:Science, LCC:Q, DOAJ:Botany, DOAJ:Biology, DOAJ:Biology and Life Sciences, LCC:Plant culture, LCC:SB1-1110, LCC:Agriculture, LCC:S, DOAJ:Plant Sciences, DOAJ:Agriculture and Food Sciences
Publisher: Grupo Paulista de Fitopatologia
Year: 2007
DOI identifier: 10.1590/S0100-54052007000100014
OAI identifier: oai:doaj.org/article:f9ab252eae1e4d83930ecc81aabb2212
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • http://doaj.org/search?source=... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.