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Microsatellite markers for identification of a group of italian olive accessions Identicação de grupos de cultivares italianos de oliva com marcadores microssatélites

By Innocenzo Muzzalupo, Francesca Stefanizzi, Amelia Salimonti, Rosanna Falabella and Enzo Perri

Abstract

Cultivar characterization for fruit trees certification requires fast, efficient and reliable techniques. Microsatellite markers (SSR) were used in the molecular characterization of 23 genotypes of Olea europaea subsp europaea. The DNA from the olive cultivars was analyzed using nine pre-selected SSR primers (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) and revealed 29 alleles, which allowed each genotype to be identified. In the dendrogram, the nine primers allowed the 23 olive genotypes to be grouped into subgroups corresponding to the same cultivar denominations. SSR markers proved to be efficient and reliable for the molecular characterization of Italian olive cultivars.<br>A caracterização de cultivares na produção de mudas certificadas exige técnicas rápidas, eficientes e confiáveis. Marcadores microssatélites (SSR) foram utilizados objetivando a caracterização molecular de 23 genótipos de Olea europaea subsp europaea. O DNA das cultivares foi analisado por meio de nove primers SSR pré-selecionados (GAPU59, GAPU71A, GAPU71B, GAPU103A, UDO99-01, UDO99-12, UDO99-28 and UDO99-39) e reveleram um total de 29 alelos que permitiram individualizar cada um dos genótipos. No dendrograma, os nove primers permitiram a separação dos 23 genótipos, em subgrupos. Os SSR foram eficientes e confiaveis para a caracterização molecular de cultivares italianeo de oliva

Topics: Olea europaea, fingerprinting, identidade genética, marcadores moleculares, Olea europaea, fingerprinting, genetic identity, molecular markers, LCC:Agriculture (General), LCC:S1-972, LCC:Agriculture, LCC:S, DOAJ:Agriculture (General), DOAJ:Agriculture and Food Sciences
Publisher: São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Year: 2009
DOI identifier: 10.1590/S0103-90162009000500014
OAI identifier: oai:doaj.org/article:e142f9337ff74bb894c7e987122093c5
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