Objective: to compare the output from a novel high power, wide separation laser Doppler flow probe (DP1-V2-HP, 4mm with IRLD20) with that of a standard flow probe (DP1-V2, 0.5mm, with DRT4) (Moor UK) and to explore its potential for use in the nonivasive measurement of blood flow in deeper tissues in humans.<br/><br/>Methods: Monte Carlo modeling was used to predict depths of light scattering in skin with each probe, geometry. Experimentally, forearm blood flow was measured at rest and during local warming of the skin surface and post occlusion reactive hyperaemia (PORH). Laser Doppler blood flus (LDF) and the power spectral density of its component frequency intervals were compared.<br/><br/>Results: Monte Carlo modeling indicated that while the majority of wide probe LD signal derives from deeper tissue, a significant portion is from superficial (dermal) tissue (and vice versa for standard probe). Perturbation of local blood flow differentially increased LDF and spectral power as measured by the two probes, with the standard skin probe showing a significantly greater response to local skin warming.<br/><br/>Conclusions: these differences support our hypothesis that the wide probe is recording predominantly blood flux within the vasculature of sub-dermal tissue. This is in agreement with Monte Carlo simulation
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