the results of SUB1 expression and transgenic rescue of the sub1 mutant, supplementary material is available on Science Online at www.sciencemag.org/ cgi/content/full/291/5503/487/DC1. The SUB1 coding region corresponding to residues 267 to 552 of the SUB1 translation product (SUB1c) was amplified by PCR, expressed, purified from Escherichia coli, and used to raise polyclonal antibodies against SUB1. The 45 Ca 2� overlay calcium-binding assay was as described (31, 34). Light sources, hypocotyl measurement, genetic and transgenic analyses, RNA blot, immunoblot, and GUS fusion protein cellular localization analyses were a
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