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Short Communication OXIDATIVE STRESS INDUCES IGF-I RECEPTOR SIGNALING DISTURBANCES IN CULTURED HUMAN DERMAL FIBROBLASTS. A POSSIBLE MECHANISM FOR COLLAGEN BIOSYNTHESIS INHIBITION

By Paweł Sienkiewicz, Maciej Pałka and Jerzy Pałka

Abstract

Abstract: The effects of oxidative stress on collagen and DNA biosynthesis, β-galactosidase and prolidase activities, and the expression of prolidase, β1integrin receptor, FAK, IGF-IR and MAP-kinases (ERK1, ERK2) were evaluated in human dermal fibroblasts. Subconfluent cells were subjected to repetitive stresses with 30µM t-BHP for 1 hour per day over the course of 5 days. It was found that oxidative stress induced the inhibition of collagen biosynthesis in these cells in a time-dependent manner. Exposure of the cells to 5 stresses contributed to a decrease in collagen and DNA biosynthesis to about 30 % and 50 % of the control values, respectively. Prolidase activity and expression were only suppressed in fibroblasts subjected to 1 and 3 stresses. In these cells prolidase activity was decreased by about 20%. As a result of 5 stresses, no further inhibition of prolidase activity occurred; however, expression of the enzyme was slightly increased, as demonstrated by Western blot analysis. It was found that these phenomena were neither related to the expression of β1-integri

Year: 2004
OAI identifier: oai:CiteSeerX.psu:10.1.1.417.817
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