Different Analytical Methods for the Determination of Nifedipine in the Tablet Formulation.

Abstract

Pharmaceutical analysis is an interdisciplinary subject and derives its principles from various branches of science like chemistry, physics, microbiology, nuclear science and electronics. Analytical determination is based on the measurement of some physical, chemical or structural properties which are related directly or indirectly to the amount of desired constituent present in the sample the methods were very simple and accurate. When compared to separation techniques such as chromatography the developed methods were simple cost effective and adequately sensitive. Hence the proposed methods were found to be convenient and suitable for adopting analysis of nifedipine in bulk drug and its formulation. Nifedipine was selected for the quenchofluorimetric determination. It is an indirect spectrofluorimetric method. So different fluorescent probe moieties could be adopted for the assay of the drug that has the quenching ability. Quinine sulphate was found to be suitable probe moiety. The experimental conditions are as follows, Excitation wavelength and emission wavelength were selected. Determination of linearity range for the drug. Checking the interference due to excipients in the assay procedure. The proposed assay procedure is validated and the accuracy of the method was established from the recovery studies and also by comparing the results with the alternate analytical method being UV-Visible spectrophotometric method. Precision of the method was tested by the reproducibility of the results and computition of % RSD which is low. Fluorescence method is being a sensitive analytical technique which has been adopted in present work. This method could be used in establishing the quality control of any formulation that contain nifedipine