Project Goals: Establish methodologies for assessing and mitigating the risks of future large-scale metabolic engineering microbial projects, including those extending beyond the bioreactor. The DOE EERE-funded Advanced Biofuel Process Development Unit (ABPDU) houses two 300 liter microbial fermentation tanks. In the extremely unlikely event that the ABPDU’s post-fermentation microbicidal protocol (e.g. base treatment and neutralization) should catastrophically fail, broth harboring viable genetically engineered micro-organisms could be purged directly to downstream waste-water treatment processes. This Ethical, Legal and Social Implications (ELSI) pilot study seeks to quantitate the risks associated with this scenario, by measuring the viability of the engineered microbes (and perhaps mor
To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.