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RESEARCH ARTICLE Human Mutation OFFICIAL JOURNAL Cystathionine b-Synthase Mutations: Effect of Mutation Topology on Folding and Activity www.hgvs.org

By Jitka Sokolová, Veronika Klatovská, Jakub Krijt, Karel Jelínek, Jan P. Kraus, Communicated David and N. Cooper

Abstract

ABSTRACT: Misfolding of mutant enzymes may play an important role in the pathogenesis of cystathionine b-synthase (CBS) deficiency. We examined properties of a series of 27 mutant variants, which together represent 70 % of known alleles observed in patients with homocystinuria due to CBS deficiency. The median amount of SDS-soluble mutant CBS polypeptides in the pellet after centrifugation of bacterial extracts was increased by 50 % compared to the wild type. Moreover, mutants formed on average only 12 % of tetramers and their median activity reached only 3 % of the wild-type enzyme. In contrast to the wild-type CBS about half of mutants were not activated by S-adenosylmethionine. Expression at 181C substantially increased the activity of five mutants in parallel with increasing the amounts of tetramers. We further analyzed the role of solvent accessibility of mutants as a determinant of their folding and activity. Buried mutations formed on average less tetramers and exhibited 23 times lower activity than the solvent exposed mutations. In summary, our results show that topology of mutations predicts in part the behavior of mutant CBS, and that misfolding may be an important and frequent pathogenic mechanism in CBS deficiency

Topics: topology, E. coli
Year: 2010
OAI identifier: oai:CiteSeerX.psu:10.1.1.352.149
Provided by: CiteSeerX
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