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Advance Access publication July 16, 2007 Transcriptional Regulation of Deoxynivalenol-Induced IL-8 Expression

By In Human Monocytes, Jennifer S. Gray and James J. Pestka


The trichothecene mycotoxin deoxynivalenol (DON), commonly present in contaminated grains worldwide, induces expression of the chemokine interleukin (IL)-8 in human monocytes. The purpose of this study was to test the hypothesis that DON modulates transcriptional and posttranscriptional regulation of IL-8 expression in the U937 human monocyte model. When U937 cells were transfected with a wild-type IL-8 promoter luciferase construct (2162/144 IL-8 LUC) and incubated with DON (1 mg/ml) or the positive control, lipopolysaccharide (LPS) (1 mg/ml), there was a significant increase in luciferase expression. Mutation of the nuclear factor-kB (NF-kB) binding site significantly impaired both DON- and LPS-induced luciferase expression. In contrast, mutating the activator protein-1 binding site resulted in significantly increased DON- and LPSinduced luciferase expression. CCAAT/enhancer-binding protei

Year: 2007
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