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Advance Access publication July 16, 2007 Transcriptional Regulation of Deoxynivalenol-Induced IL-8 Expression

By In Human Monocytes, Jennifer S. Gray and James J. Pestka

Abstract

The trichothecene mycotoxin deoxynivalenol (DON), commonly present in contaminated grains worldwide, induces expression of the chemokine interleukin (IL)-8 in human monocytes. The purpose of this study was to test the hypothesis that DON modulates transcriptional and posttranscriptional regulation of IL-8 expression in the U937 human monocyte model. When U937 cells were transfected with a wild-type IL-8 promoter luciferase construct (2162/144 IL-8 LUC) and incubated with DON (1 mg/ml) or the positive control, lipopolysaccharide (LPS) (1 mg/ml), there was a significant increase in luciferase expression. Mutation of the nuclear factor-kB (NF-kB) binding site significantly impaired both DON- and LPS-induced luciferase expression. In contrast, mutating the activator protein-1 binding site resulted in significantly increased DON- and LPSinduced luciferase expression. CCAAT/enhancer-binding protei

Year: 2007
OAI identifier: oai:CiteSeerX.psu:10.1.1.322.3787
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