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Intragraft I Cell Receptor Transcript Expression in Human Renal Allografts1

By Martha Pavlakis, Mark L. Lipman, Terry B. Strom, M. Paviakis, Ml. Lipman, Tb. Strom and Department Of


Allograft rejection is a T cell-dependent process. It is not known whether rejection is mediated by a limited number of T cell clones or by a polyclonal population of I cells. Several studies attempting to answer this question using molecular techniques to analyze the T cell receptor (TCR) population have reached conflicting conclusions. Reverse transcription-assisted polymerase chain reaction (PCR) has been used to quantify T cell infiltration and examine TCR heterogeneity in kidney transplant biopsies from patients experiencing graft dysfunction. RNA from snap-frozen biopsies gathered on 23 transplant patients was reverse transcribed to cDNA and used as the template for PCR. The constant region gene of the TCR beta chain (Cf3), 2

Year: 2013
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