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Vitamin D Receptor mRNA Measured in Leukocytes with the TaqMan Fluorogenic Detection System: Effect of Calcitriol Administration

By Genetics, Laura Soldati, Donatella Adamo, Cristiana Bianchin, Teresa Arcidiacono, Annalisa Terranegra, Maria Luisa Bianchi, Stefano Mora and Daniele Cusi

Abstract

Background: The aim of the present study was to investigate the interactions between the circulating concentrations of 1,25-dihydroxyvitamin D 3 [1,25(OH) 2D 3] and the mRNA concentration of its specific nuclear receptor in human leukocytes. Methods: We measured vitamin D receptor (VDR) mRNA extracted from leukocytes by use of TaqMan fluorescence analysis applied to the reverse transcription-PCR (RT-PCR) technique in 16 volunteers before and after calcitriol administration. VDR mRNA was also measured in leukocytes from calcium-stone-formers (37 hypercalciuric and 34 normocalciuric patients). The relationship between VDR mRNA concentrations and genetic VDR polymorphisms was analyzed in these patients. Results: Imprecision (CV) of RT-PCR was 1.3 % within assay (n � 10) and 1.7 % between assays (n � 4). Ora

Year: 2004
OAI identifier: oai:CiteSeerX.psu:10.1.1.321.8693
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