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Characterization of rabies virus nucleocapsids and recombinant nucleocapsid-like structures

By Annie Barge, Florence Baudin, Danielle Blondel and Rob W. H. Ruigrok


Rabies virus nucleoprotein (N) was produced in insect cells using the baculovirus expression system described by Pre � haud et al. (Virology 178, 486– 497, 1990). The protein was either purified on a CsCl gradient, resulting in a mixture of nucleocapsid-like structures and beaded rings, as observed by electron microscopy, or on a glycerol gradient that resulted in a preparation of the rings only. The rings and nucleocapsid-like structures had the same morphological characteristics as viral nucleocapsids. N in these structures is an 84 A � long and thin molecule that is spaced at around 34 A� along the length of the nucleocapsid, identical in shape and spacing as the nucleoprotein in nucleocapsids of rabies virus and very similar to those o

Year: 1998
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