Rabies virus nucleoprotein (N) was produced in insect cells using the baculovirus expression system described by Pre � haud et al. (Virology 178, 486– 497, 1990). The protein was either purified on a CsCl gradient, resulting in a mixture of nucleocapsid-like structures and beaded rings, as observed by electron microscopy, or on a glycerol gradient that resulted in a preparation of the rings only. The rings and nucleocapsid-like structures had the same morphological characteristics as viral nucleocapsids. N in these structures is an 84 A � long and thin molecule that is spaced at around 34 A� along the length of the nucleocapsid, identical in shape and spacing as the nucleoprotein in nucleocapsids of rabies virus and very similar to those o
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