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Low-Voltage-Activated Ca 2 � Currents Are Generated by Members of the Ca vT Subunit Family (�1G/H) in Rat Primary Sensory Neurons

By Régis C. Lambert, Frank Mckenna, Yves Maulet, Edmund M. Talley, Douglas A. Bayliss, Leanne L. Cribbs, Jung-ha Lee, Edward Perez-reyes and Anne Feltz


Recently, two members of a new family of Ca 2 � channel �1 subunits, �1G (or Ca vT.1) and �1H (or Ca vT.2), have been cloned and expressed. These �1 subunits generate Ba 2 � currents similar to the T-type Ca 2 � currents present in sensory neurons. Here, we use three methods to investigate whether the T currents of nodosus ganglion neurons are encoded by members of the Ca vT family. PCR detected the presence of mRNA encoding both �1G and �1H, as well as a third highly related sequence, �1I. In situ hybridizations performed on nodosus ganglia demonstrate a high expression of �1H subunit RNAs. Transfection of nodosus ganglion neurons with a generic antisense oligonucleotide against this new �1 subunit family selectively suppresses the low-voltage-activated Ca 2 � current. The antisense oligonucleotide effect increased with time after transfection and reached a maximum 3 d after treatment, indicatin

Year: 1998
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