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www.elsevier.com/locate/ybbrc Molecular force spectroscopy of homophilic nectin-1 interactions

By Sri Ram, Krishna Vedula, Tong Seng Lim A, Shi Hui D, P. Jaya Kausalya C, E. Birgitte Lane B, Gunaretnam Rajagopal A, Walter Hunziker C and Chwee Teck Lim D

Abstract

Nectins are Ca 2+ independent cell adhesion molecules localizing at the cadherin based adherens junctions. In this study, we have used atomic force microscopy to study interaction of a chimera of extra cellular fragment of nectin-1 and Fc of human IgG (nef-1) with wild type L-fibroblasts that express endogenous nectin-1 to elucidate the biophysical characteristics of homophilic nectin-1 trans-interactions at the level of single molecule. Bond strength distribution revealed three distinct bound states (or configurations) of trans-interactions between paired nectins, where each bound state has a unique unstressed off-rate and reactive compliance. Kinetic analysis of force-dependent off-rate of the bound state involving trans-interacting V–V domains between paired nectin-1 (unstressed off-rate 1.465 ± 0.779 s 1, reactive compliance 0.143 ± 0.072 nm) was found to be closest to E-cadherin, indicating that V–V domain trans-interactions are probably necessary to initiate and promote adhesions of E-cadherin at adherens junctions (AJs)

Topics: Nectins, Cell adhesion, Single-molecule force spectroscopy
Year: 2007
OAI identifier: oai:CiteSeerX.psu:10.1.1.321.1623
Provided by: CiteSeerX
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