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Activation of LDL receptor gene expression in HepG2 cells by hepatocyte growth factor

By Youngmi K. Pak, T Matthew P. Buck, David Berries, Michael R. Brig, Allen D. Cooper and Jeff L. Ellsworth


Abstract The effect of recombinant human hepatocyte growth factor (HGF) on low density lipoprotein (LDL) receptor gene expression was studied in the human hepatoma cell line HepG2. HepG2 cells were incubated with serum-free media in the presence and absence of HGF for various times and 1251-labeled LDL specific binding at 4"C, uptake at 37"C, and the levels of LDL receptor mRNA were measured. Incubation with HGF roduced time- and concentrationdependent increases in y251-labeled LDL binding (2-fold), uptake (2.5-fold), and LDL receptor mRNA (&fold). HGF increased the rate of LDL receptor gene transcription 4- to 5-fold relative to that of several "house-keeping " genes as measured by nuclear run-on transcription. The half-life of LDL receptor mRNA, measured with actinomycin D, was not increased in HGF-treated cells. The stimulation of LDL receptor expressio

Year: 2013
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