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Printed in U.S.A. Copyright © 1999 by The Endocrine Society

By Normal Prostate, Epithelial Cells, Cervix Cancer and Cell Lines

Abstract

The 3�-hydroxysteroid dehydrogenase/isomerase (3�-HSD) isoenzymes catalyze an essential step in the formation of all classes of active steroid hormones. In humans there are two 3�-HSD isoenzymes, the type 1 gene being predominantly expressed in the placenta and peripheral tissues, whereas the type 2 gene is the predominant 3�-HSD expressed in the adrenal glands and gonads. We have recently showed that interleukin (IL)-4 and IL-13 induce 3�-HSD type 1 gene expression in human breast cancer cell lines as well as in normal human mammary epithelial cells. The present study was designed to investigate whether such a cytokine-induced 3�-HSD type 1 expression would also be observed in cell types derived from other peripheral sex steroid target tissues. To gain further knowledge about the molecular mechanism of IL-4 action, we have studied whether the induction of 3�-HSD type 1 expression in IL-4-responsive cell types would always be associated with the activation of Stat6, a member of the Signal Transducers and Activators of Transcription (STAT) gene family. Stat6 is recognized as the principal transcription factor mediating the effects of IL-4. In normal human prostate epithelial cells (PrEC), no 3�-HSD activity was detectable under basal culture conditions, while exposure to IL-4 or IL-13 caused a poten

Year: 1999
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