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Dynamic localization of the nuclear import receptor and its interactions with transport factors

By Darren H. Wong, Anita H. Corbett and Pamela A. Silver


Abstract. Characterization of the interactions between soluble factors required for nuclear transport is key to understanding the process of nuclear trafficking. Using a synthetic lethal screen with the rnal-1 strain, we have identified a genetic interaction between Rnalp, a GTPase activating protein required for nuclear transport, and yeast importin-[3, a component of the nuclear localization signal receptor. By the use of fusion proteins, we demonstrate that Rnalp physically interacts with importin-~. Mutants in importin- ~ exhibit in vivo nuclear protein import defects, and importin-[3 localizes T RANSPORT of proteins from the cytoplasm to the nucleus is a regulated process that depends not only on an intrinsic signal in the protein to be imported

Year: 1996
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