Abstract. Characterization of the interactions between soluble factors required for nuclear transport is key to understanding the process of nuclear trafficking. Using a synthetic lethal screen with the rnal-1 strain, we have identified a genetic interaction between Rnalp, a GTPase activating protein required for nuclear transport, and yeast importin-[3, a component of the nuclear localization signal receptor. By the use of fusion proteins, we demonstrate that Rnalp physically interacts with importin-~. Mutants in importin- ~ exhibit in vivo nuclear protein import defects, and importin-[3 localizes T RANSPORT of proteins from the cytoplasm to the nucleus is a regulated process that depends not only on an intrinsic signal in the protein to be imported
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