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PKA-dependent Regulation of mKvl.l, a Mouse Shaker-like Potassium Channel Gene, When Stably Expressed in CHO Cells

By Martha M. Bosma, Margaret L. Allen, Troy M. Martin and Bruce L Tempel


Potassium (K) channels are important regulators of cellular physiology and can themselves be modulated by phosphorylation. We have investigated the potential protein kinase A (PKA) regulation of mKv1.l, a mouse Shaker-like K channel gene, when it is expressed in stably transfected Chinese hamster ovary (CHO) cell lines. Whole-cell patchclamp records show that expression of mKvl.l gives rise to a rapidly activating, sustained K+ current, referred to classically as a delayed rectifier-type current. In order to study the effects of PKA, we compared cell lines transfected with mKv1.l alone with lines cotransfected with both mKv1.l and a plasmid encoding a dominant negative mutation in the regulatory subunit of PKA. These mutant regulatory subunits bind to endogenous catalytic subunits of PKA but do not respond to CAMP, thereby causing a chronic reduction in th

Year: 2013
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