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ORIGINAL ARTICLE Adipose Tissue Exosome-Like Vesicles Mediate Activation of Macrophage-Induced Insulin Resistance

By Zhong-bin Deng, Anton Poliakov, Robert W. Hardy, Ronald Clements, Cunren Liu, Yuelong Liu, Jianhua Wang, Xiaoyu Xiang, Shuangqin Zhang, Xiaoying Zhuang, An V. Shah, Dongmei Sun, Sue Michalek, William E. Grizzle, Timothy Garvey, Jim Mobley and Huang-ge Zhang

Abstract

OBJECTIVE—We sought to determine whether exosome-like vesicles (ELVs) released from adipose tissue play a role in activation of macrophages and subsequent development of insulin resistance in a mouse model. RESEARCH DESIGN AND METHODS—ELVs released from adipose tissue were purified by sucrose gradient centrifugation and labeled with green fluorescent dye and then intravenously injected into B6 ob/ob mice (obese model) or B6 mice fed a high-fat diet. The effects of injected ELVs on the activation of macrophages were determined through analysis of activation markers by fluorescence-activated cell sorter and induction of inflammatory cytokines using an ELISA. Glucose tolerance and insulin tolerance were also evaluated. Similarly, B6 mice with different gene knockouts including TLR2, TLR4, MyD88, and Toll-interleukin-1 receptor (TIR) domain–containing adapto

Year: 2013
OAI identifier: oai:CiteSeerX.psu:10.1.1.319.9931
Provided by: CiteSeerX
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