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Biological and biochemical characterization of a cloned Leu-3 cell surviving infection with the acquired immunodeficiency syndrome retrovirus

By M. Folks, Douglas Powell, Marilyn Lightfoote, Scott Koenig, Anthony S. Fauci, Steven Benn, Arnold Rabson, Daryl Daugherty, Howard E. Gendelman, M. David Hoggan, Sundararajan Venkatesan and Malcolm A. Martin

Abstract

A unique human retrovirus (RV) 1 has been consistently isolated from patients with the acquired immune deficiency syndrome (AIDS). Infection of human T lymphocytes or T lymphocyte lines with the AIDS RV can result in a variety of outcomes ranging from rapid cell death to the integration of functionally inert proviral DNAs (1-3). A typical AIDS RV infection is characterized by the appearance of multinucleated cells, a burst of reverse transcriptase (RT) activity, and profound cellular degeneration that extends over a 5-20-d period (1, 4). Two recent reports have described noncytotoxic effects of the AIDS RV on human lymphocytes. In one case (2), PHA-stimulated, IL-2-dependent cultures of helper-inducer human T cells exhibited the characteristic cytopathic effects of acute viral infection, but nonetheless, sufficient numbers of cells survived to continue to produce infectious virus during the 4 mo they were maintained in culture. In the other (3), we described Leu-3- non-virus-producing ceils, derived from a Leu-3 + T cell line, that survived infection with the AIDS RV, and which could be induced with 5-iodo-2'-deoxyuridine (IUdR) to express infectious viru

Year: 1986
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